Human pluripotent stem cells (hPSCs) are a vital tool not only in research and disease modeling but also in developmental biology and regenerative medicine because of their ability to self-renew and differentiate. Current genome editing tactics in hPSCs require the incorporation of deleterious double-stranded DNA breaks followed by inefficient homology-directed...

In recent years, the true power of predictable and high-fidelity genome engineering for applications in gene therapy and biotechnology is closer than ever to being realized, and the CRISPR and CRISPR-associated (Cas) systems have played an instrumental role in this development. While CRISPR offers great programmability, it isn’t perfect and its...