Human pluripotent stem cells (hPSCs) are a vital tool not only in research and disease modeling but also in developmental biology and regenerative medicine because of their ability to self-renew and differentiate. Current genome editing tactics in hPSCs require the incorporation of deleterious double-stranded DNA breaks followed by inefficient homology-directed...

Accurate detection and diagnosis of infectious diseases is paramount to appropriate treatment and better outcomes. Despite tremendous efforts to advance new diagnostic techniques, there are still critical gaps in the healthcare system in quickly diagnosing and responding to infectious diseases. Current diagnostic technologies identify pathogens by detecting...

Targeting DNA manipulation is integral to biotechnology and synthetic biology. Large chromosomal rearrangements and deletions have profound impacts on bacterial physiology, such as improved bioproduct levels, increased strain fitness, or changed tolerance to stress and environmental conditions. However, it requires time-consuming and laborious directed...

In recent years, the true power of predictable and high-fidelity genome engineering for applications in gene therapy and biotechnology is closer than ever to being realized, and the CRISPR and CRISPR-associated (Cas) systems have played an instrumental role in this development. While CRISPR offers great programmability, it isn’t perfect and its...