Single-cell proteomics is an emerging field for proteomic analyses of individual cells. Single-cell genomic analyses have greatly expanded our understanding of cell heterogeneity and disease progression; however, protein identification and quantitative cell-to-cell variation provide a deeper level of understanding of the biological regulators in the...

Background Serial femtosecond crystallography (SFX) with recent advances in X-ray free electron lasers (XFELs) is a revolutionary technique for determining the structure of macromolecules at ambient temperature. It has enabled the study of large membrane protein complexes at atomic resolution and their reaction dynamics, previously considered impossible...

Background Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) has enabled crystal structure determination of reaction intermediates at ambient temperatures. Unlike traditional crystallography, where a single crystal is irradiated as it rotates, SFX usually involves the injection of a continuous stream of randomly oriented...