Nanoarray-based assays offer advantages over standard microarray assays such as minimal sample consumption, high efficiency, and enhanced multiplexing capacity. However, despite their potential, they face challenges such as the complexity and high cost of spotting and fabrication, as well as a lack of effective detection schemes. This impedes many practical...

The US Government’s pursuit of the “Thousand Dollar Genome” and the “X Prize” goal of even lower cost DNA sequencing are striking examples of the importance being assigned to advances in methods for the DNA sequencing of the human genome. The first technology that finally allows for truly routine genome sequencing will...

The use of monoclonal antibodies (mAbs) in research, diagnostics and therapeutics is ubiquitous, particularly because of their ability to simultaneously achieve high affinity and specificity. mAbs binding typically involves interactions between it and 5-8 amino acids in a specific spatial arrangement. These interactions at defined positions is what...

T cell receptors (TCRs) play an important role in the adaptive immune system. The ability to accurately predict or characterize binding interactions between TCRs and target antigens, partly because of the vast diversity of TCRs and epitopes, is challenging. While there has been progress in the development of computational models dedicated to this, the...

Valley Fever (VF), or coccidioidomycosis, is a systemic fungal infection caused by the fungus Coccidioides that is endemic to the Southwestern United States but can occur in other regions of the world. Roughly 40% of VF patients experience vague or flu-like symptoms and approximately 5-10% of patients, progress to a disseminated form of the disease...

Major histocompatibility complex class I (MHC-I) molecules are principal components of immune recognition. Peptide binding to MHC-I molecules is necessary for antigen presentation and T cell recognition. Understanding and predicting MHC-I binding has great utility in T cell therapies, vaccine development and treatment of complex human diseases. Unfortunately,...

Accurate detection and diagnosis of infectious diseases is paramount to appropriate treatment and better outcomes. Despite tremendous efforts to advance new diagnostic techniques, there are still critical gaps in the healthcare system in quickly diagnosing and responding to infectious diseases. Current diagnostic technologies identify pathogens by detecting...

Accurate detection and diagnosis of infectious diseases is paramount to appropriate treatment and better outcomes. Despite tremendous efforts to advance new diagnostic techniques, there are still critical gaps in the healthcare system in quickly diagnosing and responding to infectious diseases. Current diagnostic technologies identify pathogens by detecting...

the second most common cancer in both men and women and the leading cause of cancer death in the US. Although there are risk factors for lung cancer, such as smoking, there are many cases where the patient never smoked. Because of this, there is growing interest in investigating the lung microbiota and how that may promote or prevent disease. Yearly...

FFPE is a well-established and widely used method for preservation and histological analysis of tissue samples. DNA/RNA/and Proteins can be extracted from FFPE samples, but generally lack single cell resolution and completely lack 3D spatial resolution. Enabling 3D resolution allows the correlation of morphology to information like gene expression,...